A Candida parapsilosis Overexpression Collection Reveals Genes Required for Pathogenesis

Relative to the vast data regarding virulence mechanisms of Candida albicans, there is limited knowledge on emerging opportunistic human pathogen parapsilosis. The aim this study was generate and characterize an overexpression mutant collection identify explore factors in C. With obtained mutants, we investigated stress tolerance, morphology switch, biofilm formation, phagocytosis, vivo Galleria mellonella larvae mouse models. In order evaluate results, compared from parapsilosis analysis results derived previous deletion library characterizations. Of 37 identified eight with altered phenotypes controls. This work first report CPAR2_107240, CPAR2_108840, CPAR2_302400, CPAR2_406400, CPAR2_602820 as contributors by regulating functions associated host-pathogen interactions formation. Our findings also confirmed role CPAR2_109520, CPAR2_200040, CPAR2_500180 pathogenesis. attempt use strategy systematically assess gene function parapsilosis, our demonstrate that approach effective for such investigations.